You can't, as far as I can tell, plus IO slotting won't help buff their defense.
I believe FFG + Maneuvers + Protectors bubbles + EofM +def should get you pretty close to all but the Protector's bots. Id on't know the numbers well enough to tell, t hough.
50s: Yumi Eryuha-Arch/Energy, Mirria-Thugs/Dark, Meyami Kitsuna-Claws/SR, Celesta Seusen-SS/Invuln, Lady Mirriella-Illusion/Empathy
Arc 503982 "Dimension Xi Epsilon 22-10" Part one of a multi-part arc.
The defense you get from the protector bots is 7.5% base and ~11% with ED capped slotting. The build I've made for my own bots/traps softcaps myself to all 3 positions with their bubble and then the T1 and T3 bots are also softcapped. The protectors, meanwhile, hover around 34% since they don't get double bubbles like the others (you don't either).
Edit: Also, while you have to do the math manually, you can get the numbers from slotting the protectors with defense. I would start a new build for a night widow and pick Foresight and then slot it. It also has a 7.5% base defense so the results from slotting will be the same.
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The Usual Suspects: Fimboolvetr (Icer Tank), Tsukiyomi (Mind/Psi/Ice Dom), Smiting Dragon (Dm/Sr Scrap), Widow Mortis (NW)
Up and Comers: Ameterasu (Km/Reg Scrap), Arrhymian (Elec/Nin Stalk), TDMKII (Bot/Traps MM)
The problem with putting in Pet buffs is that I haven't yet figured out a way to do it automatically - it keeps getting lost every time we create a new database. I have plans for doing an overhaul of the Pet system in Mids, but it comes after PVP DR and I21.
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After two years, I finally got my bots/traps to 50 and I'm ready to slot him with IO sets. My question is how do I see my pets' stats in Mids. I like to try and softcap their defense if possible and in the process softcap my MM's defense but also include pet buffs (protector's bubbles) so I don't go over cap and waste slots.